Assessment of Levonorgestrel Leaching in a Landfill and Its Effects on Placental Cell Lines and Sperm Cells

The Buenavista landfill is located east of the city Medellín, but it has a slope steeper than 30% and less 600 m away from Piedras River, possibly influencing quality drinking water in city. Many complex residues are disposed this landfill, including pharmaceuticals personal care products (PPCPs) such as levonorgestrel (LNG), which may reach bodies via runoff leaching. We assessed levels LNG effluent an upflow anaerobic sludge blanket (UASB) reactor by uHPLC–DAD, well endocrine disruptor effect on placental cell lines (BeWo) human sperm cells. Additionally, potential leaching was assayed under laboratory conditions using soil layers that were sampled landfill. detected at 315 ?g·L?1 effluents UASB reactor. Thus, not efficient treatment method for removal recalcitrant pollutants. we found layer used cover material adsorb more 90% pollutants, small amounts still be leached, means strong enough barrier to fully prevent LNG. Finally, our results show leachate fraction decreased ?-human chorionic gonadotropin, motility or viability. leached could trigger reproduction disorders, further studies should carried out investigate its effects detail.